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Laboratory Techniques

Laboratory Techniques resources.

RIAlity

Describes the basics of radio immuno assay (RIA), a technique for measuring concentrations of antigens. 

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Separating proteins using SDS Page

Articulate presentation detailing the theory and practice of protein separation using SDS PAGE. This unit includes a video demonstration of how to set up and run an SDS PAGE gel.

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Techniques in Molecular Biology

Articulate presentation with quizzes describing basic techniques in molecular biology with emphasis on the use of plasmids as cloning vectors.

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Enzyme Assays

Articulate presentation illustrating the basic principles of enzyme assays, including spectrophotometry and the Michaelis-Menten equation.

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pH and Buffers

Articulate presentation explaining pH, the effective use of indicators and how to make up and use buffers correctly, including a worked example of how to make up some standard buffer solutions.

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Ion Exchange Chromatography

Articulate presentation describing the process of protein separation using ion exchange chromatography, illustrated using animations and a video demonstration of how to set up an ion exchange chromatography column.

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Spectrophotometry

A video introduction to the spectrophotometer and principles of the Bradford Assay for determining protein concentration.

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Introduction to Microscopy

A short video demonstrating how to set up a light microscope.

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The Gram Stain

Video demonstration of how to conduct a Gram stain to identify bacterial types.

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Basic Microbiology Techniques

Video introduction to asceptic techniques and preparing agar plates for incubation.

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The Haematoxylin and Eosin Stain

A series of 8 videos providing an overview and then step by step guide to the Haematoxylin and Eosin stain.

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