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biochemistry

Molecular Separation

29th March 2016

An articulate presentation describing biochemical techniques used for molecular separation. Topics covered include pH and buffers, molecular exclusion chromatography, ion exchange chromatography, affinity chromatography and SDS PAGE. These techniques are described using both text and video demonstrations. The resource was created by Dr Sue Whittle & Dr Sue Bickerdike, University of Leeds and can be downloaded

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pH and Buffers

29th March 2016

An articulate presentation explaining pH, the effective use of indicators and how to make up and use buffers correctly. It also contains a worked example of how to make up some standard buffer solutions. The resource was created by Dr Sue Whittle & Dr Sue Bickerdike, University of Leeds and can be downloaded here.

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Spectrophotometry – principles and practice

29th March 2016

An articulate presentation detailing the theory behind the use of spectrophotometers to detect and quantify biological molecules. Further to this the Beer-Lambert Law is explored and methods to establish standard curves for calibration and determination of unknown concentrations explained, as is the method for the determination of the peak wavelength of a sample. The presentation […]

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Enzyme Assays

29th March 2016

This articulate presentation illustrates the basic principles of enzyme assays. The resource describes how to use spectrophotometry to measure reaction rates, the effect on substrate concentration on reaction rates and explains the principles behind the Michaelis-Menten equation. The resource was created by Dr Sue Whittle & Dr Sue Bickerdike, University of Leeds and can be downloaded here.

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Techniques in Molecular Biology

29th March 2016

This articulate presentation describes some of the basic techniques in molecular biology with particular emphasis on the use of plasmids as cloning vectors. The theory behind the techniques used to isolate plasmid DNA, carrying out restriction digests, pcr and running agarose gels is described in detail, supported by animations to illustrate the text. Quizzes are […]

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The Citric Acid Cycle

23rd September 2015

This resource is an interactive website designed to help students tackle The Citric Acid Cycle. Complete with detailed information, animations and an interactive quiz, this assists students in one of the complex and essential biochemical cycles in the human body. The resource can be accessed from here. The resource was created by the University of Stanford […]

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Web Anatomy: Self Tests

23rd September 2015

Web Anatomy: Self Tests Web Anatomy: Self Tests is a selection of online quizzes aimed at various aspects of physiology and it’s underlying biochemistry. Quizzes are in a variety of different formats including: MCQ, Blank Space and Drag and Drop for different ways of reinforcing the information.  Physiological topics include: Histology, Integumentary System, Skeletal System,  Muscular System, Nervous System, Endocrine System,  Cardiovascular […]

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